Construction of improved plasmid vectors for promoter characterization inPseudomonas aeruginosaand other Gram-negative bacteria
نویسندگان
چکیده
منابع مشابه
Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli.
A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519nfp, broa...
متن کاملConstruction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.
We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.
متن کاملDevelopment of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria.
Full exploitation of the information available in bacterial genome sequences requires the availability of facile tools for rapid genetic manipulation. One bacterium for which new genetic tools are needed is the methylotroph Methylobacterium extorquens AM1. IncQ and small IncP vectors were shown to be unsuitable for use in this bacterium, but a spontaneous mutant of a small IncP plasmid was isol...
متن کاملSynthesis and Characterization of Novel Antibacterial PDDA/Honey Nanofiber Against Gram-Positive and Gram-Negative Bacteria
Nanomaterials are increasingly used to the targeting of gram-positive and gram-negative bacteria as an alternative to antibiotics. Bacterial infections are a major cause of chronic infections and mortality. People requirement for new materials for pathogenic bacteria treatment. It seems that nanomaterial-based strategies can be resolving this problem. In this research, improved antibacterial na...
متن کاملASD-GFP vectors for in vivo expression technology in Pseudomonas aeruginosa and other gram-negative bacteria.
We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate beta-semialdehyde dehydrogenase (asd) gene as a selectable marker and beta-galactosidase (pIVPRO, 10.88 kb) or mutant green fl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1998
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1998.tb13315.x